Viable Plate Counting. Viable Plate Count (also called a Standard Plate Count) is one of the most common methods, for the enumeration of bacteria. Serial dilutions of bacteria are plated onto an agar plate. Either by spread plate method or pour plate method. The dilution procedure influences the overall counting process. The total plate count (TPC) in the original sample is calculated by multiplying the number of CPUs with the respective dilution factors. The ‘rules of enumeration’ are followed, while calculating the number of bacteria in the original sample. The most common method for determining the total viable count is the pour-plate method. The pour plate technique can be used to determine the number of microbes/ mL in a specimen. It has the advantage of not requiring previously prepared plates and is often used to assay bacterial contamination of foodstuffs. SimPlate for Total Plate Count (TPC) method is used for the detection and quantification of the total aerobic microorganisms in food. The medium/sample mixture is dispensed into a SimPlate device and incubated for 24-28 h. The total aerobic plate count is determined by counting the fluorescent wells and referring to the SimPlate Conversion Table. I have followed the same protocol of total plate count except changes in media. 40 colony is obtained in 10-3 dilution factor. 40 x 1000/0.1= log 4 x 10 5 = 5.6. For total plate count count, the The number of colonies we count on a plate gives us the CFU or colony forming units, when we divide the CFU, by the volume we plated we get CFU/volume à CFU/mL. For example, if we counted 32 bacteria on a plate that had received 200 μl of a 10-6 dilution, Total Volume (Ratio) Individual Dilution . Factor. Total Dilution. Factor. SimPlate Total Plate Count Color Indicator (TPC-CI) method is used for the detection and quantification of total aerobic microorganism populations. It is based on Binary Detection Technology (BDT) which equates the presence of total aerobic microorganisms to the presence of a color change in the medium. Aerobic plate count. The aerobic plate count (APC) is also known as standard plate count, aerobic mesophilic count, total plate count or aerobic colony count. The APC is used to estimate the bacterial population in a food sample. The total plate count is the enumeration of aerobic, mesophillic organisms that grow in aerobic conditions under moderate temperatures of 20-45°C. This includes all aerobic bacteria, yeast, molds and fungi that grows in the specific agar. Plate counting method Step One: Diluting the sample. Depending on the source of the sample used there might be thousands, millions or even billions of microorganisms per millilitre of sample. This is too many for us to count so we dilute the sample. 1ml of sample is added to 9ml of a suitable diluent (e.g. sterile buffer) (Figure 1a).
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Total plate count - Duration: 8:06. Sudheendra C ... Most Probable Number (MPN) Method - Duration: 7:05. Biology Professor 40,443 views. 7:05. Basic Pour∕Spread Plate Reportable ... This video describes the process of serially diluting bacteria from a soil sample and then enumerating colonies (cfu's) on either Nutrient or MacConkey agar. MICROORGANISMS TESTING IN WATER Hi, Everyone. We are UTHM students from Food Technology course. This video is a Laboratory Exercise Food Microbiology For Education Purpose. Sign in to make your opinion count. Sign in. 3 1. Don't like this video? Sign in to make your opinion count. ... Total plate count - Duration: 8:06. Sudheendra C 58,521 views. 8:06. Language: ... Total plate count - Duration: 8:06 ... Total Bacterial Count for Water analysis ... Venkatesh Suriya Ch 3,153 views. 9:56. Serial Dilution Method Protocol Step Wise Explanation ... Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube.
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